| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.049298 |
| Chromosome: | chromosome 6 |
| Location: | 1775879 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263200 | FAP325 | Flagellar Associated Protein 325 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTACCGCAGAAGAACATCCATCCGCGCGGCAGGCCGCAGTTAGTGAC |
| Internal bar code: | GCGTAGACCCTATGAGGTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2880 |
| LEAP-Seq percent confirming: | 58.3333 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCAAGCCCCGTATAGGAC |
| Suggested primer 2: | CATGCAAGCAACCGTGTGAA |