Insertion junction: CLIP2.049327_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.049327
Chromosome:chromosome 2
Location:7150259
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g390319 (1 of 1) PTHR10663:SF42 - PROTEIN BOI2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTTTAAGACTTGAGGCCCCCAGAATATTTAATATATTTTACTTTTTAAG
Internal bar code:GATAACTGATTAATGTCTTATT

Confirmation - LEAP-Seq

LEAP-Seq distance:2211
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GAATGCCGCAAGATCGTGTC
Suggested primer 2:GCGGTGCTGTCAGAGTACTT