Insertion junction: CLIP2.049331_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.049331
Chromosome:chromosome 1
Location:5295058
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g037150 CLV6 (1 of 1) PTHR11689//PTHR11689:SF14 - CHLORIDE CHANNEL // CHLORIDE CHANNEL PROTEIN 2; ion channel/ voltage-gated chloride channel CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGACGTCGTGTGGCCGCGTCTCCGTCACACGCGTTGCCGCTGCGCACA
Internal bar code:TATGCACATGCGGTTGATGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:1341
LEAP-Seq percent confirming:85.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:AACTCCACCACACACACCTC
Suggested primer 2:GAGGCAAAGTCCTAGGCGTT