| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049335 |
| Chromosome: | chromosome 12 |
| Location: | 4711983 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522700 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGTCTTGTCATTGGCAGGCGTCCCGGCACGGCCTCATCGTGGCAATGC |
| Internal bar code: | AATGTACGCTGTAGTCAGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2253 |
| LEAP-Seq percent confirming: | 75.3425 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGAGATGGTTGCGTGCT |
| Suggested primer 2: | ACCTCCCACTCCCTGTCTTT |