| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.049339 |
| Chromosome: | chromosome 2 |
| Location: | 8375494 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g142650 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCGAGTTACTGACTATGTGCGATGCAACTGGACAGAGTCCCTGGGGAG |
| Internal bar code: | ATACGATAGAGATGTCGTTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2548 |
| LEAP-Seq percent confirming: | 77.2727 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTCCCCTACGTTCTCCCT |
| Suggested primer 2: | GTTAGCTGGTCCGTGGACAA |