Insertion junction: CLIP2.049344_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.049344
Chromosome:chromosome 10
Location:2440066
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g435350 ESU1,ESU1a Endosulfine cAMP-regulated phosphoprotein; (1 of 2) PF04667 - cAMP-regulated phosphoprotein/endosulfine conserved region (Endosulfine) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTACACGGACCCGCCGCTGAGGCAGCCCCGAGAAGCTGAAACGAGTACG
Internal bar code:AAAGCATGCATTTGCGACATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1981
LEAP-Seq percent confirming:70.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGCGTAGGCCTACTTCTC
Suggested primer 2:ATGCACACGGAGGTCAGAAG