Insertion junction: CLIP2.049351_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.049351
Chromosome:chromosome 2
Location:4802793
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g109000 (1 of 2) PF03254 - Xyloglucan fucosyltransferase (XG_FTase) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGACGTGCTGGTAGAACTCCACTGTCGACTGGGGCGGGAACAGCAGCGC
Internal bar code:TATGTTCACGACTCCTGGTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3661
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTTCTGTTACGGGTGGAA
Suggested primer 2:CCACTCCCCATCAACAGCAT