Insertion junction: CLIP2.049358_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.049358
Chromosome:chromosome 2
Location:1135753
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g080800 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTCCCCGCCGCGCCCAGCGCCGCAGCCGCCGCTGTCGTGGACACCAGG
Internal bar code:AATAGACCTGTGTAGGTCCCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:639
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAAGTCACTTTGCGGTGG
Suggested primer 2:GGCGGCAGTAGCAGTAGTAG