| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049377 |
| Chromosome: | chromosome 3 |
| Location: | 6843067 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197500 | MOT6 | (1 of 1) PTHR10891//PTHR10891:SF554 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN // SUBFAMILY NOT NAMED; Predicted protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTCATTCACCTTCATTTTACAGTCGCTCCAAACCGCTTCTACCGCTG |
| Internal bar code: | TTCATATTACATGCTGGGACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4688 |
| LEAP-Seq percent confirming: | 94.8276 |
| LEAP-Seq n confirming: | 110 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 116 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACAGGTATAGCGGGGGA |
| Suggested primer 2: | TGGGAATTAGCTGCACCCTG |