Insertion junction: CLIP2.049378_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.049378
Chromosome:chromosome 13
Location:2602942
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g580800 (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGCGGCGGCGAGTACGGAGGCGGTAGCGACAGCGAGGATGACGAGCC
Internal bar code:ACGCATGAATGGTGTGACTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:5085
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCCATCGAACCAGCTCAA
Suggested primer 2:TGCGAAGACATGGACGACAA