| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.049389 |
| Chromosome: | chromosome 3 |
| Location: | 6151466 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190850 | ECH1 | EnoylCoA hydratase/isomerase; (1 of 1) K13238 - 3,2-trans-enoyl-CoA isomerase, mitochondrial (DCI) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTGTTGCTGGACAGCCGCCGCATAGCGCCCTCCAGCACCTTGATTGT |
| Internal bar code: | GCTCTGAGCTAAAAGACGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3034 |
| LEAP-Seq percent confirming: | 65.3465 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 101 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCTAAATTCTGGGGGCT |
| Suggested primer 2: | GTAGTTACGCCGACTACCCG |