| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049408 |
| Chromosome: | chromosome 17 |
| Location: | 1821591 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709300 | (1 of 1) PF10009 - Uncharacterized protein conserved in bacteria (DUF2252) (DUF2252) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGGCGAGGCGTCGACGGGCGGCCTTGCTGAGTTCGGGTCCGGTGCGA |
| Internal bar code: | CGCCTCGGTAGGAAGAACAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2769 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTGCAGATGGAGCATGC |
| Suggested primer 2: | TGATGATGGACTGGTTGGCC |