| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.049409 |
| Chromosome: | chromosome 12 |
| Location: | 4848637 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523600 | (1 of 3) PTHR13367//PTHR13367:SF8 - TUMOR NECROSIS FACTOR-RELATED // OTU DOMAIN-CONTAINING PROTEIN 7B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGAGACGCTGAGGAAGGTATGGATGAAGCGCGGCTCCTCAAATTTGG |
| Internal bar code: | CGCATAGGATGAAGAGTTCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3480 |
| LEAP-Seq percent confirming: | 94.8276 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGCCCAAGGACATTACT |
| Suggested primer 2: | ACGCTGACTGCTACCTTTCC |