Insertion junction: CLIP2.049414_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.049414
Chromosome:chromosome 17
Location:5439265
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g738800 (1 of 1) PTHR15537:SF2 - F-BOX ONLY PROTEIN 7 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACCACACTCCACACCCCCCCACACACACACACACATACACACAC
Internal bar code:CACTATGTTCTCACATAACGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2690
LEAP-Seq percent confirming:95.6522
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:GCGAGGAGTACAGTACGTCG
Suggested primer 2:CATCGACCTGTCCGAGTACG