| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049416 |
| Chromosome: | chromosome 7 |
| Location: | 945333 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319150 | PTP4 | Protein tyrosine phosphatase; (1 of 2) PTHR10367 - MRNA-CAPPING ENZYME | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGATTAGACAGATGTGTTTTGGTGCGTGTTCTTAATGAAGTTAATGAA |
| Internal bar code: | CCGAAGGTCGGTACGTTGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 510 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAGGTAGCAGCACACCAC |
| Suggested primer 2: | GTGTGTGAGGACCTGACCAG |