| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.049416 |
| Chromosome: | chromosome 7 |
| Location: | 945337 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319150 | PTP4 | Protein tyrosine phosphatase; (1 of 2) PTHR10367 - MRNA-CAPPING ENZYME | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTGTCCTGCGCGTATTGTTCTCCATGAGCTCTTCAACATATACACACA |
| Internal bar code: | CTATCGACAGTAATGAACTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 267 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGAGGACCTGACCAG |
| Suggested primer 2: | ATCAGGTAGCAGCACACCAC |