Insertion junction: CLIP2.049425_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.049425
Chromosome:chromosome 4
Location:1217950
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g215250 FAP26 Ankyrin Repeat Flagellar Associated Protein 26; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCAAGGACGCAGCAACAACAACGGAGGGAGGGTGTTACACACATGAGC
Internal bar code:CTTTGCGCTATGTTTAGATTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:79
LEAP-Seq percent confirming:40.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTGCGCTTATTACACAGC
Suggested primer 2:CCAGTTTGTTTGATGGCGCA