| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.049427 |
| Chromosome: | chromosome 12 |
| Location: | 5840510 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533250 | (1 of 2) IPR000104//IPR001394//IPR028889 - Antifreeze protein, type I // Peptidase C19, ubiquitin carboxyl-terminal hydrolase // Ubiquitin specific protease domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACAAGGGACAAGGTATTAGGAGGCGGGAGAGGATTGGGTGGAAGTGGC |
| Internal bar code: | GCGGGTGATCGTGCGCCGAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 411 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAAGACCAAGACCAAGGA |
| Suggested primer 2: | CATCAGACGCCGAGGAAGAG |