Insertion junction: CLIP2.049433_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.049433
Chromosome:chromosome 2
Location:3236733
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre02.g097100 (1 of 8) PF01697 - Glycosyltransferase family 92 (Glyco_transf_92) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGAGACATTGGCGCGCGCAATGTTACGTGATAATGGCGGCAACGGTGGC
Internal bar code:TTGATGTACATGGACGAATCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:671
LEAP-Seq percent confirming:29.4118
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:48
LEAP-Seq n unique pos:68

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGACCACACGTAGCAACT
Suggested primer 2:CCAACGGCCCTTCTTCTTCT