| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049434 |
| Chromosome: | chromosome 2 |
| Location: | 3098660 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096050 | (1 of 2) PTHR30545:SF2 - SUGAR FERMENTATION STIMULATION PROTEIN A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCGCAGCTGCGCTCACGCCTGGGCATTCGCTCCTTGTAATCCTTAAG |
| Internal bar code: | GTATTATAGGTGTGAGTTGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1363 |
| LEAP-Seq percent confirming: | 47.0588 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCAATGCCAAGGTGAGGG |
| Suggested primer 2: | CCATTGCGCAAGACACAACA |