| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.049436 |
| Chromosome: | chromosome 12 |
| Location: | 4983221 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524950 | ENGB,ENGB1 | Factor possbly involved in ribosomal 50S subunit assembly; (1 of 1) PTHR11649//PTHR11649:SF49 - MSS1/TRME-RELATED GTP-BINDING PROTEIN // SUBFAMILY NOT NAMED | 3'UTR |
| Cre12.g525000 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | 5'UTR | |
| Cre12.g801417 | (1 of 41) PTHR10157 - DOPAMINE BETA HYDROXYLASE RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCTTACTCCTTAGCAGCAAACTCTGGCGATATCCCTCCTCCATATGTA |
| Internal bar code: | GGGTGTTCGAACACCGATTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 7.14286 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTTCATTACGCGGCATG |
| Suggested primer 2: | ATTCTCCCCACGTGTGTGTC |