Insertion junction: CLIP2.049442_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.049442
Chromosome:chromosome 16
Location:2084314
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g657450 MPA12 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) 3.6.1.13//3.6.1.16//3.6.1.53 - ADP-ribose diphosphatase / ADPR-PPase // CDP-glycerol diphosphatase / CDP-glycerol pyrophosphatase // Mn(2+)-dependent ADP-ribose/CDP-alcohol diphosphatase / Mn(2+)-dependent ADP-ribose/CDP-alcohol pyrophosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCGCCCGCACCTGCCGCTGCGCTGCTTTCAGCCGCTCCACGGTCTTCA
Internal bar code:GGTGGCTGTCAATGGAAGTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1433
LEAP-Seq percent confirming:95.8333
LEAP-Seq n confirming:69
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:72

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCAGAAGACAGCAACTCT
Suggested primer 2:GTGCCTTGGTCCAGGACTAC