| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049442 |
| Chromosome: | chromosome 16 |
| Location: | 2084337 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657450 | MPA12 | Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) 3.6.1.13//3.6.1.16//3.6.1.53 - ADP-ribose diphosphatase / ADPR-PPase // CDP-glycerol diphosphatase / CDP-glycerol pyrophosphatase // Mn(2+)-dependent ADP-ribose/CDP-alcohol diphosphatase / Mn(2+)-dependent ADP-ribose/CDP-alcohol pyrophosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGCGATCAGAGGATTGTCGGGGGCTGATGAGGGACGCCGGGGCTAGA |
| Internal bar code: | TGCGGTATACTTAGTAAAATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3336 |
| LEAP-Seq percent confirming: | 88.2353 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCTTGGTCCAGGACTAC |
| Suggested primer 2: | GGGCAGAAGACAGCAACTCT |