| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049471 |
| Chromosome: | chromosome 4 |
| Location: | 1811901 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g800464 | (1 of 24) IPR006311 - Twin-arginine translocation pathway, signal sequence | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGCAGCGGCGGCAGTAGCAGCGGCGGCGGCAGTGGCTCCGTGACCA |
| Internal bar code: | GTGATCCTCGCCTGCCAATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4141 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACACACACACACACACAC |
| Suggested primer 2: | ACCCAGACCCAGACCTACTC |