Insertion junction: CLIP2.049475_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.049475
Chromosome:chromosome 3
Location:6130540
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g800355 (1 of 3) PF03732//PF13650 - Retrotransposon gag protein (Retrotrans_gag) // Aspartyl protease (Asp_protease_2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCTGGCGCGGCTGCTGATCGCGAGCTTCTGTCACCGTCGCCACCGGCA
Internal bar code:GTTATAATTAACGGGTTGAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:2014
LEAP-Seq percent confirming:46.6667
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGTGGCAGACGATGTCTT
Suggested primer 2:TACCGTATGGAGCCATTCGC