| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.049482 |
| Chromosome: | chromosome 3 |
| Location: | 7619444 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204500 | CSE17 | Precursor of chemotactic amidated peptide | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTAATAAGCAAGTTGGTGACGGGACCCAAATACGCAAGACAGGACTAA |
| Internal bar code: | GTAGTCTTGGGATTATAAATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1681 |
| LEAP-Seq percent confirming: | 64.1026 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCCCATCCTTCAGGCTCG |
| Suggested primer 2: | CTAGGTGGCTGTGGGAACAG |