Insertion junction: CLIP2.049489_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.049489
Chromosome:chromosome 9
Location:4559620
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g401256 (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATATAGTCGTCCACCGTGGTGACATTCACATCTGCGTGCGTGCGTGTGT
Internal bar code:TTAGTTTGGCGAGCTTACGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:201
LEAP-Seq percent confirming:28.5714
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CTTTAACACGCACCCGCAAA
Suggested primer 2:AAGAAGCAGCTGTAGCCCAG