| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049494 |
| Chromosome: | chromosome 1 |
| Location: | 3842368 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025200 | MMP4 | Metalloproteinase of VMP family; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTACACGTTGTACAAGTAGTTGGGGATCCAGTCAGGCACAACCTGTAT |
| Internal bar code: | TGAGGTTGCTCAGCGAGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3472 |
| LEAP-Seq percent confirming: | 97.9167 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAATACCGAGGCGATGC |
| Suggested primer 2: | CTTGCGGTCCTTGTACTGGT |