| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.049495 |
| Chromosome: | chromosome 10 |
| Location: | 2415601 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435200 | (1 of 4) IPR000533 - Tropomyosin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCGTCCTGATGCAGCCTCTCCTCCTTCTTGCGCAGGTCCGTGCGCAGC |
| Internal bar code: | GTTACAGGATCAAACGGTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4610 |
| LEAP-Seq percent confirming: | 92.1053 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTGGAGAAGGCAGAAGC |
| Suggested primer 2: | GTGTACGTGGCGTCCTTGTA |