| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049495 |
| Chromosome: | chromosome 10 |
| Location: | 2415653 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435200 | (1 of 4) IPR000533 - Tropomyosin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTGATGGGCGTTTTTGCCGAGCTCTAACGCTTAGATTACGCTGCTGC |
| Internal bar code: | ACATATTGTTTTTCAGTAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3713 |
| LEAP-Seq percent confirming: | 85.1852 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTACGTGGCGTCCTTGTA |
| Suggested primer 2: | AAGGTGGAGAAGGCAGAAGC |