| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049504 |
| Chromosome: | chromosome 14 |
| Location: | 4109835 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633750 | IPB2 | (1 of 1) PTHR10527:SF5 - FI07923P; Importin beta-3 homolog | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGCCGGGAGCTGGTCGACATCGTCAGTAACGCTGATGTTCCGCAAGA |
| Internal bar code: | GAGAGTTCCGGGTCTTGGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 366 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACGTTTGCGAAGTGCTTG |
| Suggested primer 2: | CCACACAAGCACCACTTTCG |