| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049521 |
| Chromosome: | chromosome 8 |
| Location: | 2262418 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370650 | PGL2 | (1 of 2) 3.1.1.31 - 6-phosphogluconolactonase; 6-phosphogluconolactonase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGGCGCGTCCATGCCCGTTTCCTTGCGGCCCGGGTACAGGCTGCCCAC |
| Internal bar code: | GTTTCTTCGGTATCATGTGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 229 |
| LEAP-Seq percent confirming: | 61.5385 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCTAGTTGAACGGTGGG |
| Suggested primer 2: | CACCAGCACTACTCCACCAG |