| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049533 |
| Chromosome: | chromosome 14 |
| Location: | 1080623 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614450 | Hypothetical protein; (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGACCCACATCGCCCAGAGCATTTCCGAAAGCACCGGAGGGTGTGGCCA |
| Internal bar code: | AACTAAAGCGGTTATACTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2287 |
| LEAP-Seq percent confirming: | 51.3158 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCATGCATTTCCGTGCCC |
| Suggested primer 2: | AGTGGGCAGAGGCAGAGATA |