| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.049537 |
| Chromosome: | chromosome 9 |
| Location: | 3536499 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396475 | (1 of 1) PTHR24202//PTHR24202:SF4//PTHR24202:SF52 - FAMILY NOT NAMED // E3 UBIQUITIN-PROTEIN LIGASE MIB2 // E3 UBIQUITIN-PROTEIN LIGASE MIB1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCACTTGAACTGCAGGCTACGGTGGCTGTTCGGCGGGAGCTGGCGCAG |
| Internal bar code: | ATGAGCGAAATATCGGCTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2225 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCATTGCTGACTCGCTTGT |
| Suggested primer 2: | TGCCCTCCTCCAATCCTACA |