Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.049539 |
Chromosome: | chromosome 7 |
Location: | 5302920 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g349900 | (1 of 8) 2.3.1.75 - Long-chain-alcohol O-fatty-acyltransferase / Wax synthase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAGAGCCACACGCACCCATTTCAACAGGACAAAACATTTACGCACGTG |
Internal bar code: | TGCCATAGGTCACATGTAAACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 154 |
LEAP-Seq percent confirming: | 19.0476 |
LEAP-Seq n confirming: | 4 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTGGTCAAGGCCACTGCTA |
Suggested primer 2: | ATGTCTGGGGCGAGACTTTG |