Insertion junction: CLIP2.049539_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.049539
Chromosome:chromosome 7
Location:5302920
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g349900 (1 of 8) 2.3.1.75 - Long-chain-alcohol O-fatty-acyltransferase / Wax synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACAGAGCCACACGCACCCATTTCAACAGGACAAAACATTTACGCACGTG
Internal bar code:TGCCATAGGTCACATGTAAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:154
LEAP-Seq percent confirming:19.0476
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGGTCAAGGCCACTGCTA
Suggested primer 2:ATGTCTGGGGCGAGACTTTG