| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049539 |
| Chromosome: | chromosome 7 |
| Location: | 5302930 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g349900 | (1 of 8) 2.3.1.75 - Long-chain-alcohol O-fatty-acyltransferase / Wax synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTGCGGCGGGCGGCTGGAGGGCAGCTACCACATGCTCGCCTTCTTCC |
| Internal bar code: | ATGATGGTTCATGGCGGGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3329 |
| LEAP-Seq percent confirming: | 80.6818 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCTGGGGCGAGACTTTG |
| Suggested primer 2: | CTTGGTCAAGGCCACTGCTA |