Insertion junction: CLIP2.049545_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.049545
Chromosome:chromosome 12
Location:381461
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g495600 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCACTGTACCGCTAGCACACCGCATAACTAACCACCCAGAGAAACCCA
Internal bar code:GTCGGAATCGAGCGGCTCGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1324
LEAP-Seq percent confirming:84.4444
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCCGAGTCGTAGCAGTAC
Suggested primer 2:GTCTCCCCACACACACAGAG