| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049561 |
| Chromosome: | chromosome 14 |
| Location: | 2774288 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g626466 | (1 of 30) PTHR24349:SF79 - NITROGEN NETWORK KINASE 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAACCCTCTCTCCCACGTGCTTTCACAACAAAACACAACCGGCAGCCG |
| Internal bar code: | ATGGTTACTGGACTAATCGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 977 |
| LEAP-Seq percent confirming: | 38.3562 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGCTGTGGTTCTGAACT |
| Suggested primer 2: | GAGGTCCAGCTCCAAGTCAC |