| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049580 |
| Chromosome: | chromosome 12 |
| Location: | 3201925 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500050 | (1 of 19) PF00397 - WW domain (WW) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGATGGCGCCGGCGGTGTGTCGGTCGGGAGGTCACGGCCCATGTTGCG |
| Internal bar code: | ATTTTACCGTTTCTGTGTCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2010 |
| LEAP-Seq percent confirming: | 98.5916 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACGTGTGCTCTTCTGTCC |
| Suggested primer 2: | CCAGCGAGAATGGGACCTTT |