| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049581 |
| Chromosome: | chromosome 3 |
| Location: | 4813047 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178100 | MOT51 | (1 of 1) PTHR10877//PTHR10877:SF116 - POLYCYSTIN-RELATED // SUBFAMILY NOT NAMED; Predicted protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTGGGGCAGTTCCAGGAGACGGACCCTATTGTGGGCGACGGCGAGCT |
| Internal bar code: | ACCGGTAGTCATAATTTCTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2521 |
| LEAP-Seq percent confirming: | 97.8495 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTGTACGGGGTAGGGTA |
| Suggested primer 2: | GTTCCACACGCTCAAGGAGA |