| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049584 |
| Chromosome: | chromosome 7 |
| Location: | 400552 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314866 | EBM4 | (1 of 9) 3.2.1.78 - Mannan endo-1,4-beta-mannosidase / Endo-1,4-mannanase; Mannan endo-1%252C4-beta-mannosidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACCGCAACCCGCCCGCCCACCTGAGCGCCTCCTGCAGGTGTGCGGCCA |
| Internal bar code: | AATTCAGCAAGCCCGGAAATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 927 |
| LEAP-Seq percent confirming: | 61.2245 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTACATGGCCAACTTCCT |
| Suggested primer 2: | GCGACACCCCCATATCCAAA |