Insertion junction: CLIP2.049587_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.049587
Chromosome:chromosome 15
Location:1256692
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g801725 NCL64 Nuclear Control of chloroplast-Like 64; (1 of 39) PF08373 - RAP domain (RAP) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGACGCCTAGCCTAACCTCTCACCTTGCCACACAGGTTGAAGGCAGCTG
Internal bar code:TCGTAACCGAAACAATTCTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2969
LEAP-Seq percent confirming:67.7419
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:AATGTTACAGTTCGCACGCG
Suggested primer 2:AGGACCTCTAGAGCCCACAG