| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049588 |
| Chromosome: | chromosome 12 |
| Location: | 4102100 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801403 | (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCGGAGCAGCGGTGGCAACAAGGCAGGCGCTGAGGCCAGTCCGCAAG |
| Internal bar code: | AAGTGCTCGAAGGCGGCTAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5869 |
| LEAP-Seq percent confirming: | 94.5946 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCCCAGAGAGTACAGGC |
| Suggested primer 2: | CATGGTGTTTGCCCTGTTGG |