| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049589 |
| Chromosome: | chromosome 2 |
| Location: | 5723772 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g116500 | CPY3 | Serine carboxypeptidase; (1 of 1) PTHR11802:SF30 - PROTEIN Y16B4A.2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCGCGCTCACGTCGCTCATCACCAGGTGGACCATATCATGGGCCACG |
| Internal bar code: | CCTCCCGCTTAAACAAGGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2222 |
| LEAP-Seq percent confirming: | 97.619 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCGATTGTCGTCTAGCC |
| Suggested primer 2: | CGATCTGCCCACCTCAAGTT |