Insertion junction: CLIP2.049601_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.049601
Chromosome:chromosome 7
Location:1456575
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g323750 HAS2 (1 of 1) K06133 - 4'-phosphopantetheinyl transferase (LYS5, acpT); Holo-[acyl-carrier-protein] synthase CDS
lncRNA_TCONS_00052104 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAAGGCAACACTGGGGTTTCCAGGGTATGCGCCCGGCCACACCGCATC
Internal bar code:GCCGGTACCACCTTGGTACTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:4325
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGATGAGCATTGGCCACACT
Suggested primer 2:TGCAGTGGGGATTCCGTAAC