| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.049612 |
| Chromosome: | chromosome 17 |
| Location: | 1747225 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708650 | HFO27,HFO20 | (1 of 33) K11254 - histone H4 (H4); Histone H4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGACCTTCCTGGAGAACGTCATCCGCGACTCGGTCACCTACACCGAGCA |
| Internal bar code: | GGAGATCGAGGTAGGACAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4579 |
| LEAP-Seq percent confirming: | 99.0826 |
| LEAP-Seq n confirming: | 108 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 109 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCTTTGGCTCAGGCAGA |
| Suggested primer 2: | TCCTTGGGCATGATGGTCAC |