Insertion junction: CLIP2.049627_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.049627
Chromosome:chromosome 6
Location:1614462
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g261600 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCCAGCAGGCACACCGCCGCCGCCGCGCACGCCGCCGCCACGCCCGAT
Internal bar code:ATGGTATGATACTGAAATATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2320
LEAP-Seq percent confirming:48.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GTACCTGTGTGTTCGCCTCA
Suggested primer 2:TCCATCATTCCCCTTGCACC