Insertion junction: CLIP2.049633_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.049633
Chromosome:chromosome 11
Location:655716
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467615 (1 of 2) IPR000742//IPR001881//IPR009030 - EGF-like domain // EGF-like calcium-binding domain // Insulin-like growth factor binding protein, N-terminal intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCTGGATGTTCTTGAACCGCTTTCCTGTCAGACCAACTGCCCAAGTCG
Internal bar code:CTGATGCTTAACTTTTTCTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1908
LEAP-Seq percent confirming:49.2308
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:33
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:AATGACGTGCTTGGGTCGAT
Suggested primer 2:TATCACCACACCACGTGTCG