| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049646 |
| Chromosome: | chromosome 6 |
| Location: | 2846759 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272150 | (1 of 1) 1.8.4.2 - Protein-disulfide reductase (glutathione) / Insulin reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTCACGCTGTCATCACTGACCGCCCAGTACCTGGCAGTGCTAGCGCTG |
| Internal bar code: | TTCAAGGATGACTTTGGGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2344 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATTTGCTCCGCCTGACTT |
| Suggested primer 2: | CTTATGTGCGCACGTTCGAG |