Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.049653 |
Chromosome: | chromosome 10 |
Location: | 1315667 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g427350 | CYP31,CYP39A2 | Cytochrome P450, CYP213 superfamily; (1 of 1) 1.14.13.79 - Ent-kaurenoic acid oxidase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGCTTCATCGCACATGAACACCGCACAGGTACCGGCGCTACGGCAGC |
Internal bar code: | CCACACTTATGGATCGCTCGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 4621 |
LEAP-Seq percent confirming: | 94.1176 |
LEAP-Seq n confirming: | 16 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGAGAGTCATGAGCGAGGAC |
Suggested primer 2: | ATGATCACCTGCCTCTCCCT |